At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. One plate carrier adapter is provided with the instrument. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 2. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Unit has several areas of damaged plastic. albicans pre. Our absorbance plate readers feature our PathCheck Sensor technology and. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. 00 USD. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 00. , Oxon, UK). TGFα release was calculated as described in the Results. Western blot. 8. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. , Sunnyvale, CA) at 490 nm. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Details. If depot service is the best option for you we will assist you with logistics to help you keep. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. Table 1. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. 00 USD. 5. 5% phosphoric acid) in microplates at room temperature for 10 min. Nitric Oxide Assay. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. Acceptor filter: 665/10 nm. Fully tested with Warranty. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). 1 mL of culture media with 10% FBS. plates can be used in the SPECTRAmax PLUS 384. Call 1-800-745-2710. The metals were preincubated with A for 5 min when added before heme. 5. 314 low lamp level warning. Acceptor: 665/10 nm. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. application or budget. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. TDP-43 stress granular cells were treated with 70% ice-cold. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Cell permeabilization. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. 0 to 4. PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The reaction mixture was incubated for 5 min. , San Jose, CA) using Softmax Pro 7 software. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Acceptor: 665nm. using SpectraMax i3 (Molecular devices, San Jose, USA). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 5 stars - 1602 reviewsThis assay typically develops in 1. 315 can't find zero order. TGFα release was calculated as described in the Results. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. Subsequently, the mixture was heated at 100 °C for 10. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Amino Acid Analysis. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. A. Fig. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. Microplate Readers. Clotting times (mean ± SD) for rSimukunin and rSV. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. ), which was proportional to the number of damaged cells. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). 3. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. used. The concentration of TNFα in the experimental. Details. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 2 software was used to measure and calculate of the bioactivity activity studies. , Sunnyvale, CA, USA) at 595 nm. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. Italic and boldface type are used for emphasis. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). ). The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. Number of flashes. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. Assay setup for a 384-well low-volume plate. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 2 were considered negative for IgM antibody. 10× trypsin 0. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. Stat Fax 3200 Microplate Reader. ): 8. Molecular Devices Spectramax 340PC instructions. , Sunnyvale CA). As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. SpectraMax Multi-Mode Microplate Readers. Spectrophotometer (SpectraMax 340). StakMax Microplate Handling Stacker. TGFα release was calculated as described in the Results. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). Also for: Spectramax 190, Spectramax 340pc384, Versamax. 306 plate air cal. Donor: 616/10 nm. Statistical analysis. Stat Fax 4700 Microstrip Reader. 00 USD. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. using SpectraMax i3 (Molecular devices, San Jose, USA). The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. The VersaMax and SpectraMax 190 read 96-well microplates. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. Lactate Dehydrogenase (LDH) Release. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. Molecular Devices Spectramax 340 Plate Reader Repair. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. All data were normalized to experimental day 0. SpectraMax Gemini EM & FLEXstation (bottom read) 1. The SpectraMax® iD3 Multi Mode Microplate . mums. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. 5 mL sample or standard solution was well mixed with 0. 30. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). 5. control. Details. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. To measure the cell biomass (g/L),. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). A 0. 2. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. ). SoftMax Pro. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Analysis of ALA by Ehrlich’s Reagent Assay. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 3 0. Samples were analyzed in triplicates. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Quick View. , Sunnyvale, CA). Exhibit with us {{>currencyLabel}} Back. 1x10 5 transduced, GFP + SK-NEP-1. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Tunable for filterless flexibility. 2 0. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . The initial rate of hydrolysis was determined using the early linear region of. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Lactate Dehydrogenase (LDH) Release. Microscopy. 3. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Fibrin clots were formed as described above with some modifications 46. Setup recommendations for SpectraMax. The ratio of red to green fluorescence intensity was calculated. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Details. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). #N207) LAL Reagent Water (Lonza cat. Products : SpectraMax Plus 384 reader,. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Wound healing assay. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . Dissolve 13. 7. Buffer (100 mmol L –1acetate buffer, pH 5. Molecular Devices’ PathCheck® sensor technology. Awareness Technology Inc. Quick View. Donor filter: 620 nm. Calculating % of total mediator release in RBL assay. All strains were tested three times and the results averaged. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. SpectraMax® Microplate Readers and SoftMax® Pro Software. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). . , Sunnyvale, CA, USA) at 595 nm. Call 1-800-745-2710. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. PerkinElmer. used in the SPECTRAmax PLUS. AoSMCs were seeded into 6-well culture plates. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The plate was covered and incubated for one hour at room temperature. This assay measures the two major sugars in wine, glucose and fructose. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. SpectraMax Plus 384 measuring instruments pdf manual download. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 0% and ± 0. 2 - 3. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. Each SpectraMax® 340 PC 384 Microplate Reader has an. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). 1 mL of culture media with 10% FBS. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. 0200-6117. 2 M DEEMM were mixed in a microtube,. Number of flashes. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. #N207) LAL Reagent Water (Lonza cat. Call 1-800-745-2710. Binding of capture antibodies to polystyrene beads and biotinylation. Inter-assay variation was prevented by testing all of the samples on the same day. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. SpectraMax 190 reads 96-well and strip-well plates. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. Data were expressed as the mean LPS-treated cells (100%) vs. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. , Sunnyvale, CA). 003 OD Stray light ≤ 0. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. Unit powers. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). 5 - 2. 304 signal level saturation. 1 mL of acetylacetone. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. Male 8 pin mini Din to Female 9D. As a common indicator of equine T cell activation, cytokine induction. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. D2. Statistical analysis. Immunofluorescence and Aggregation Analysis. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Please refer to photos for further. The. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). The reaction mixture was incubated for 5 min. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). No power cord or accessories included. , Fremont, CA, USA). The Catalina. The. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. DNA extraction from blood meal ELISA homogenate. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. 305 reference level saturation. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 2. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. 4. One plate carrier adapter is provided with the instrument. Analysis of ALA by Ehrlich’s Reagent Assay. Molecular Devices SpectraMAX 340 Microplate Reader. control or celatrol-treated cells. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Values were determined according to a standard curve (log/log curve-fit). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 305 reference level saturation. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. ATI/240, 250. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. In vivo anti-malarial assay in mice. 5 ng/ml; estradiol: 10 pg/ml. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Immunofluorescence and Aggregation Analysis. 7. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. 5. SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. Service parts for these models are not being produced and are no longer in stock. Awareness Technology, Inc. 2. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 30. Endpoint, kinetic, spectrum. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Awareness Technology, Inc. Bioz Stars score: 86/100, based on 1 PubMed citations. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Molecular Device M2e. Intra- and inter-assay coefficients of variation were lower than 8. Bertin Precellys Evolution Touch Homogenizer. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Summary of Contents for Molecular Devices SPECTRAmax 340PC. Cytotoxicity was determined by measuring the release of LDH. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. 313 reference gain check fail. using SpectraMax i3 (Molecular devices, San Jose, USA). It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. 1: SpectraMax Plus 384 components. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. N-34-LB. Molecular Devices Corporation 1311. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates.